Phytochemical Characterization and Total Phenolic Content Analysis of Aqueous Leaf Extract of Vernonia elaeagnifolia (Curtain Creeper)

By K. D. Shinde1, D. H. Pawar1, P. M. Agale1, J. C. Chaudhari2, P. S. Ahire2, Dr. R. A. Ahirrao3

1P. G. College of Pharmaceutical Science and Research, Nandurbar (MS), India
2Assistant Professor, Same Institution
3Principal, P. G. College of Pharmaceutical Science and Research

Abstract

Vernonia elaeagnifolia is an ornamental climber with limited phytochemical exploration. This study evaluated its aqueous leaf extract for phytochemical composition and total phenolic content. Screening revealed alkaloids, flavonoids, tannins, proteins, and phenolics. Total phenolic content was 2490.49 µg/mL GAE. HPLC confirmed gallic acid and catechin, indicating antioxidant and potential anticancer activity.

Keywords:

Vernonia elaeagnifolia, total phenolic content, gallic acid, HPLC, antioxidant potential

Introduction

Vernonia elaeagnifolia (curtain creeper) is a fast-growing climber from the Asteraceae family, widely used for ornamental and environmental purposes. Despite its ecological importance, its phytochemical and medicinal properties remain underexplored. Phenolic compounds such as gallic acid contribute to antioxidant and anticancer activity.

Materials and Methods

Leaves were collected from Nandurbar, India, shade-dried, and extracted using aqueous maceration. Phytochemical screening and total phenolic content were determined using standard protocols, and HPLC analysis was performed using a C18 column.

Results

Phytochemical screening confirmed the presence of carbohydrates, alkaloids, proteins, saponins, flavonoids, tannins, phenolics, and amino acids, while steroids were absent.

Total phenolic content showed strong calibration linearity (R² = 0.9774) with values around 85.36 mg GAE/g extract and 34.28 µg/mL in solution.

HPLC analysis revealed gallic acid as a major phenolic compound, with concentrations ranging from 554 to 2490.50 µg/mL based on peak area analysis.

Discussion

The results confirm Vernonia elaeagnifolia as a rich source of phenolic compounds. The presence of gallic acid and catechin supports antioxidant and anticancer potential through apoptosis induction and oxidative stress reduction.

Conclusion

The aqueous extract of Vernonia elaeagnifolia shows significant phenolic content and promising therapeutic potential. Further in vitro and in vivo studies are necessary to confirm its biological activity.

References

  1. Kattekola P. (2020). Vernonia elaeagnifolia review.
  2. Wang P. et al. (2022). Urban greening systems.
  3. Sultana A. et al. (2017). Pharmacological evaluation.
  4. Additional references as per original article.