Phytochemical Characterization and Quantitative Analysis of Total Phenolic Content in Cymbopogon citratus Leaves: A Preclinical Assessment for Anticancer Potential

By K. J. Pawar1, M. P. Rathod1, R. Y. Sonar1, J. C. Chaudhari2, P. S. Ahire2, Priyanka Soni3, R. A. Ahirrao4*

1P. G. College of Pharmaceutical Science and Research, Nandurbar (MS), India
2Assistant Professor, Same Institution
3B.R. Nahta College of Pharmacy, Mandsaur University, India
4Principal, P. G. College of Pharmaceutical Science and Research

Abstract

Cymbopogon citratus (lemongrass) is widely used in traditional medicine due to its bioactive compounds, especially phenolics. This study conducted phytochemical screening, quantified total phenolic content using the Folin–Ciocalteu method, and identified compounds via RP-HPLC. Results confirmed the presence of alkaloids, flavonoids, tannins, and proteins. Total phenolic content ranged from 163.7–251.4 mg GAE/100 g extract, with gallic acid identified as a major component. These findings support its antioxidant and potential anticancer properties.

Keywords:

Cymbopogon citratus, phenolic compounds, gallic acid, HPLC, antioxidant, anticancer potential

Introduction

Cymbopogon citratus is a medicinal plant widely used for its antimicrobial, antioxidant, and anti-inflammatory properties. Phenolic compounds such as gallic acid play a key role in reducing oxidative stress and cancer risk.

Materials and Methods

Leaves were collected from Nandurbar district, India. Aqueous extraction was performed using cold maceration. Phytochemical screening included tests for alkaloids, flavonoids, tannins, proteins, and carbohydrates.

Results

Phytochemical analysis confirmed the presence of major bioactive compounds including alkaloids, flavonoids, tannins, carbohydrates, and proteins, while saponins and steroids were absent.

Total phenolic content was determined using a gallic acid calibration curve showing strong linearity (R² = 0.9774).

HPLC analysis identified gallic acid as a major phenolic compound with concentration approximately 20 µg/mL.

Discussion

The findings align with previous studies confirming high phenolic content in lemongrass. Gallic acid contributes to antioxidant and anticancer activity by inducing apoptosis and inhibiting cancer cell proliferation.

Conclusion

Cymbopogon citratus contains significant phenolic compounds and shows promise for anticancer applications. Further in vitro and in vivo studies are required to validate its therapeutic potential.

References

  1. Shah G. et al. (2011). Therapeutic uses of Cymbopogon citratus.
  2. Hadidi M. et al. (2024). Health benefits of gallic acid.
  3. Shaikh JR. (2020). Phytochemical screening methods.
  4. Additional references as per original article.