Introduction
Tinospora cordifolia (Giloy) is a medicinal plant widely used in Ayurveda for its immunomodulatory and rejuvenating properties. It contains alkaloids, glycosides, phenolics, and diterpenoid lactones, contributing to antioxidant and anticancer activity. Studies have shown cytotoxic effects against cancer cell lines, supporting its potential as a natural anticancer agent.
Materials and Methods
Leaves were collected from Nandurbar, India, authenticated, shade-dried, and extracted using aqueous maceration. Phytochemical screening was conducted using standard qualitative tests. Total phenolic content was determined using the Folin–Ciocalteu method, and HPLC analysis was performed using a C18 column for phenolic profiling.
Results
Phytochemical screening confirmed the presence of alkaloids, carbohydrates, flavonoids, tannins, saponins, proteins, and phenolic compounds.
Total phenolic content was calculated using a gallic acid calibration curve (R² = 0.9774), indicating strong antioxidant potential.
HPLC analysis showed gallic acid concentrations corresponding to peak areas:
| Gallic Acid (µg/mL) | Peak Area |
|---|---|
| 5 | 554 |
| 10 | 1066.1457 |
| 15 | 1499.0721 |
| 20 | 1953.7331 |
| 25 | 2490.4974 |
Discussion
The presence of phenolic compounds, especially gallic acid, supports the antioxidant and anticancer potential of Tinospora cordifolia. These compounds contribute to apoptosis induction and free radical scavenging activity.
Conclusion
Tinospora cordifolia demonstrates promising anticancer potential due to its phenolic content. Further research is required to validate its therapeutic applications and mechanism of action.
References
- Jagetia G.C. (2019). Anticancer activity of Tinospora cordifolia.
- Ahmad R. et al. (2015). In vitro anticancer activity studies.
- Mishra A. et al. (2013). Medicinal efficacy of Tinospora cordifolia.
- Additional references as per original article.